Error Correction in Excitation-Emission Matrices Using Principal Component Method

Fluorescence spectroscopy that uses excitation wavelength scanning is a powerful chemical, biological and medical diagnostic tool, that makes it possible to characterize the distribution of fluorophores in samples of various nature. However, when the fluorophores are unknown from the start, it greatly complicates the interpretation of large sets of excitation wavelength-dependent fluorescence spectra, especially if the measured excitation-emission matrices contain data losses due to random causes or systematic factors. It is proposed to use the principal component method to restore damaged spectral regions. Since the fluorescence spectra of individual fluorophores do not depend on the excitation wavelength, it is possible to do this without data loss.