The Immunostimulant Role Of Broccoli Sprouts As a Dietary Supplement On The Adverse of DMBA In Mice.

This study evaluates the immunostimulant and protective effects of broccoli sprouts and their bioactive compound, sulforaphane (SFN), against 7,12-dimethylbenz[a]anthracene (DMBA)-induced toxicity in mice. DMBA, a potent polycyclic aromatic hydrocarbon carcinogen, causes immunosuppression, inflammation, tumor formation, and weight loss. Fifty male mice were divided into five groups: negative control (N.C.), positive control exposed to DMBA (P.C.), and three treatment groups receiving SFN at 20, 25, and 30 mg/kg. SFN was administered orally for eight weeks. Results showed that SFN dose-dependently reduced weight loss and tumor incidence, with the highest dose lowering tumor formation by 78%. Immunoglobulin levels (IgG, IgM, IgA), suppressed by DMBA, were significantly restored in SFN-treated mice, especially at 30 mg/kg. Furthermore, IL-6 gene expression—markedly elevated in the P.C. group—was downregulated by up to 68.9% in the SFN 30 group. Histopathological analysis confirmed reduced tissue dysplasia in SFN groups. These findings suggest that broccoli sprouts and sulforaphane enhance antioxidant defenses and modulate immune responses, offering a promising dietary intervention for populations exposed to environmental carcinogens. The study supports the incorporation of broccoli sprouts as a functional food with chemopreventive and immunoprotective benefits.