Effects of Acetamiprid Exposure on Complete Blood Count (CBC) Parameters in Pregnant Mice

This study was conducted to investigate the potential effects of acetamiprid (ACP), a neonicotinoid insecticide, on the complete blood count (CBC) of pregnant mice. The widespread use of acetamiprid in agricultural practices has raised concerns regarding its safety for non-target species, particularly mammals (Phogat et al., 2022). Thirty pregnant mice were used and divided into three groups: a control group (10 mice), a low-dose group (10 mice, 5 mg/kg acetamiprid), and a high-dose group (10 mice, 25 mg/kg acetamiprid). The mice were exposed to acetamiprid via oral gavage daily throughout the gestation period. The CBC of the pregnant mice was analyzed at 21 days to assess the persistent effects of prenatal acetamiprid exposure. The results showed significant alterations in some CBC parameters compared to the reference ranges for pregnant mice. Statistical analysis was performed using one-way ANOVA and Tukey's test to compare the means between groups. Key findings included a significant dose-dependent decrease in hemoglobin levels and white blood cell counts, alongside a notable increase in platelet counts. These findings contribute to the understanding that prenatal exposure to acetamiprid at doses of 5 and 25 mg/kg may lead to significant alterations in the CBC of pregnant mice, indicating potential adverse health effects that persist postnatally. The results highlight the need for further research to understand the underlying mechanisms of these effects and assess the potential long-term risks of acetamiprid exposure during pregnancy.