CpG-oligodeoxynucleotides challenged macrophages ameliorate acetaminophen induced liver injury by activating TLR9/IRG1/itaconate metabolic pathway
CpG ODN alleviated liver injury induced by APAP through the activation of the TLR9/NF-κB signaling pathway in macrophages, upregulating the expression of IRG1 protein, promoting the production of endogenous metabolite itaconate, and inhibiting macrophage apoptosis which was regulated by upregulating the expression of Nrf2 to inhibit ROS production. This study sheds new light on CpG ODN as a therapeutic strategy in resistance to APAP-induced liver injury.
Authors
- Jun Zhou (ORCID: https://orcid.org/0000-0001-9655-6588)
- Si-Dan Luo
- Haihua Luo (ORCID: https://orcid.org/0000-0003-4607-9917)
- Kai Wu (ORCID: https://orcid.org/0000-0002-5016-0251)
- Zhixia Chen (ORCID: https://orcid.org/0000-0002-5830-1485)
- Gang Yuan (ORCID: https://orcid.org/0000-0001-7715-8787)
- Y. Zhang (ORCID: https://orcid.org/0000-0002-3657-4255)
- Zhengzheng Yan (ORCID: https://orcid.org/0000-0003-3082-6762)
- Yanxia Gao (ORCID: https://orcid.org/0000-0001-5054-6624)
- Yibai Qu (ORCID: https://orcid.org/0000-0001-7621-1996)
- Bingyao Xie
- Shan Li (ORCID: https://orcid.org/0000-0002-9995-989X)
- Li Chen (ORCID: https://orcid.org/0000-0001-9372-5606)
- Tian Tian (ORCID: https://orcid.org/0000-0002-4137-5015)
- Quan Li (ORCID: https://orcid.org/0000-0001-8563-1802)
- Yuan Ding (ORCID: https://orcid.org/0000-0002-9165-7467)
- Xubo Wu
- Yong Jiang (ORCID: https://orcid.org/0000-0002-0163-5089)
- Zehui Jiang
Institutions
- Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
- Fudan University (CN)
- Dongguan University of Technology (CN)
- Third Affiliated Hospital of Southern Medical University (CN)
- Henan Academy of Sciences (CN)
- Minhang District Central Hospital (CN)
- Dongguan People’s Hospital (CN)
- Henan Cancer Hospital (CN)
- First Affiliated Hospital of Zhengzhou University (CN)
- Southern Medical University (CN)
Publication Details
- Journal
- Molecular Medicine
- Published
- 2025-08-25
- DOI
- https://doi.org/10.1186/s10020-025-01324-0
- Citations
- 3
- Primary Topic
- Drug-Induced Hepatotoxicity and Protection
- Type
- article
- Field-Weighted Citation Impact
- 4.67