Development and application of a NP-cELISA for the detection of nucleoprotein antibodies of equine influenza virus

Equine influenza (EI) is a highly contagious respiratory disease that poses significant economic and health challenges to the global equine industry. Current diagnostic methods, such as hemagglutination inhibition (HI), are accurate but complex and impractical for widespread use, especially in regions like China where commercial kits are unavailable. This study developed a competitive ELISA (cELISA) for detecting EI virus antibodies, offering a simpler, faster, and more cost-effective alternative. The assay demonstrated higher concordance with HI than existing commercial kits and effectively monitored antibody responses in vaccinated horses. Additionally, it enabled the first large-scale serological survey of EI in China, providing critical insights into the virus's prevalence. This advancement supports timely disease detection and control, benefiting veterinary practices and the equine industry worldwide.

Authors

Institutions

Publication Details

Journal
Microbiology Spectrum
Published
2025-08-25
DOI
https://doi.org/10.1128/spectrum.00939-25
Primary Topic
Influenza Virus Research Studies
Type
article
Field-Weighted Citation Impact
0.00

Funders

Controls
|||
ALL TIME
JAN
FEB
MAR
APR
MAY
JUN
JUL
AUG
SEP
OCT
article

Development and application of a NP-cELISA for the detection of nucleoprotein antibodies of equine influenza virus

Wei Guo, Zhe Hu, Xiaoyu Chu, Zhenyu Zhang et al.
Microbiology Spectrum
Influenza Virus Research Studies
article

Development and application of a NP-cELISA for the detection of nucleoprotein antibodies of equine influenza virus

Wei Guo, Zhe Hu, Xiaoyu Chu, Zhenyu Zhang, Kui Guo, Zenan Zhang, Shuaijie Li, Xiaojun Wang, Ling Xu, Mingqi Dong, Wen Liu, Yaoxin Wang, Yan Yang
article en

Abstract

Equine influenza (EI) is a highly contagious respiratory disease that poses significant economic and health challenges to the global equine industry. Current diagnostic methods, such as hemagglutination inhibition (HI), are accurate but complex and impractical for widespread use, especially in regions like China where commercial kits are unavailable. This study developed a competitive ELISA (cELISA) for detecting EI virus antibodies, offering a simpler, faster, and more cost-effective alternative. The assay demonstrated higher concordance with HI than existing commercial kits and effectively monitored antibody responses in vaccinated horses. Additionally, it enabled the first large-scale serological survey of EI in China, providing critical insights into the virus's prevalence. This advancement supports timely disease detection and control, benefiting veterinary practices and the equine industry worldwide.

Microbiology SpectrumVol. 13(10)
University of Wisconsin–Madison (US), Xinjiang Academy of Agricultural Sciences (CN), Harbin Electric Corporation (China) (CN), Chinese Academy of Agricultural Sciences (CN), Harbin Veterinary Research Institute (CN)
Natural Science Foundation of Heilongjiang Province
Openalex Percentile: Top 70%
Influenza Virus Research Studies
DOIOpen Access PDFOpenAlex
AI Navigator

Ask Laika to Summarize, Analyze, and Connect papers live on the map.

Summarize Papers & Methodologies

Extract key findings, datasets, and comparative methods across publications.

Benchmark Rankings & Visual Analytics

Rank top research institutions, authors, funders, topics, and journals by Field-Weighted Citation Impact (FWCI) and paper volume with instant charts.

Connect Distant Disciplines

Bridge topological clusters on the map to find hidden collaborative intersections.