Association of rs1544410 Polymorphism VDR gene with Recurrent Pregnancy Loss in Iraqi Patients Women

Objective: Recurrent loss of pregnancy is one of among the most prevalent clinical events that occur in the initial, second, and thrid trimesters. Therefore, while this research aims to assess the connection between the vitamin D receptors gene (V.D.R) SNPs (rs1544410) and the risk of recurrent pregnancy loss. Methods: The cross-sectional research focused on fifty women with recurrent pregnancy loss and 50 control women without history of pregnancy failure. We applied the polymerase chain reaction (P.C.R) techniques to amplify the polymorphism areas of the V.D.R gene on each chromosome 12. The P.C.R products of every specimen were purified using the Wizard SV Gel and PCR Clean-Up System (Promega, USA) and forwarded for sequencing using the Sanger sequencing method at Macrogen, Korea. All DNA sequences were compared to the reference genome sequence of the VDR gene. Results: Our results showed the case patient group consisted of 15(30 %) CC wild type, 29(58%) CT mutant heterozygote, and 6(12%) TT homozygote genotypes of rs1544410 in VDR gene, while the control included 25(50%) CC wild type, 21(42%) CT heterozygote, and 4(8%) TT homozygote genotypes. There was a significant decrease in CC genotype in recurrent pregnancy compared with control group of dominant model (P=0.04), and significant association of rs1544410 polymorphism and VD3, WBC, RBC, GOT, GPT and Diastolic Blood Pressure. Conclusion: The present research found a substantial association between rs1544410 variations of the V.D.R gene and recurrent abortion, and significant association of rs1544410 polymorphism with study parameters.