Comparison of current methods for genome-wide DNA methylation profiling
Our findings underscore the strengths and limitations of current DNA methylation detection methods. EM-seq and ONT emerge as robust alternatives to WGBS and EPIC, offering unique advantages: EM-seq delivers consistent and uniform coverage, while ONT excels in long-range methylation profiling and access to challenging genomic regions. These insights provide practical guidance for method selection based on specific experimental goals.
Authors
- Guy Van Camp (ORCID: https://orcid.org/0000-0001-5105-9000)
- Ken Op de Beeck (ORCID: https://orcid.org/0000-0001-5527-6838)
- Ana Regina de Abreu (ORCID: https://orcid.org/0000-0001-6830-7891)
- Ligia Mateiu (ORCID: https://orcid.org/0000-0002-2655-3581)
- Joe Ibrahim (ORCID: https://orcid.org/0000-0001-9728-5273)
- Vasileios Lemonidis
Institutions
- University of Antwerp (BE)
- Antwerp University Hospital (BE)
Publication Details
- Journal
- Epigenetics & Chromatin
- Published
- 2025-08-25
- DOI
- https://doi.org/10.1186/s13072-025-00616-3
- Citations
- 11
- Primary Topic
- Epigenetics and DNA Methylation
- Type
- article
- Field-Weighted Citation Impact
- 8.87
Funders
- Fonds Wetenschappelijk Onderzoek
- Stichting Tegen Kanker
- Universiteit Antwerpen
- Vlaamse regering