Optimized in vivo two-photon imaging reveals the essential role of the contralateral eye in functional optic nerve regeneration in zebrafish larvae
Our optimized imaging platform visualizes the entire visual pathway and cell interactions during regeneration, revealing contralateral eye is essential for functional recovery following optic nerve transection. Combined with multi-omics and calcium imaging, this approach potentially provides a powerful platform to decipher the cellular and molecular mechanisms of zebrafish eye-brain pathway reconstruction and offers insights into therapeutic targets for human optic neuropathies.
Authors
- Wentao Yan (ORCID: https://orcid.org/0000-0001-8480-6375)
- Yikui Zhang (ORCID: https://orcid.org/0000-0001-9060-602X)
- Y. Edward Wen (ORCID: https://orcid.org/0000-0002-7376-0014)
- Bin Xie (ORCID: https://orcid.org/0000-0003-2980-8583)
- Ya Gao (ORCID: https://orcid.org/0000-0001-8087-1364)
- Wencan Wu (ORCID: https://orcid.org/0000-0002-6229-373X)
- Baoguo Shen (ORCID: https://orcid.org/0000-0003-3886-355X)
- Hongyuan Wei (ORCID: https://orcid.org/0000-0003-2069-1841)
- Siyu Dong (ORCID: https://orcid.org/0009-0006-5042-5981)
- Tao Yang (ORCID: https://orcid.org/0000-0003-1273-0089)
- Lanfang Sun (ORCID: https://orcid.org/0000-0002-3990-7051)
- Ting Li (ORCID: https://orcid.org/0000-0002-0461-0880)
- Geng Yuan
- Ziwen Chen (ORCID: https://orcid.org/0000-0001-5278-7819)
Institutions
- Wenzhou Medical University (CN)
- Affiliated Eye Hospital of Wenzhou Medical College (CN)
- Zhejiang Lab (CN)
Publication Details
- Journal
- Eye and Vision
- Published
- 2025-08-25
- DOI
- https://doi.org/10.1186/s40662-025-00447-z
- Citations
- 1
- Primary Topic
- Photoreceptor and optogenetics research
- Type
- article
- Field-Weighted Citation Impact
- 0.91
Funders
- National Natural Science Foundation of China
- China Postdoctoral Science Foundation
- National Key Research and Development Program of China