Simultaneous Measurement of Enzyme Activity and Concentration via a Multiplexed Photonic Sensor

Enzyme assays are a cornerstone of basic biology and clinical diagnostics. While enzymatic assays are frequently used to indicate disease,[1] changes in enzyme activity can occur due to inhibitor or activator agents[2] without changing the concentration of the enzyme. ELISA assays can be used for quantification of concentration, but the technique must be performed separately and takes several hours[3]. To address this issue, we have developed the first multiplexed photonic biosensor able to measure enzyme activity and concentration simultaneously.