Structural Biomedical Two-Photon Microscopy Using a Sub-35 fs, High-Energy (>40 nJ), Pre-Chirp Managed Yb:Fiber Laser System

Two-photon microscopy (2PM) is a vital imaging tool for modern life science [1]. As enabling technology, compact Yb:fiber lasers combined with novel labelling techniques have emerged as rapidly advancing sources for high-performance biomedical 2PM applications [2]. Here, we demonstrate a novel, highly-optimized Yb:fiber laser (YFL) design that uses simulation-guided nonlinear amplification to generate pulses with up to 40 nJ pulse energy at sub-35 fs duration for multimodal two-photon imaging of labelled mouse kidney and protein crystals.