Advancing Histopathology with Multiplex Stimulated Raman Scattering
Stimulated Raman Scattering (SRS) enables label-free, chemically specific imaging by detecting the vibrational properties of tissues. Recently, Stimulated Raman Histology (SRH) has gained prominence as a label-free imaging alternative for visualizing histopathological features and enabling quantitative evaluation [1], [2]. However, current SRH methods [3] are restricted to fresh or frozen samples, as they rely on the simultaneous acquisition of only two Raman peaks at ~2845 cm<sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">−1</sup> and 2930 cm<sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">−1</sup> to maximize contrast between nuclei and cytoplasm.
Authors
- Eric Michele Fantuzzi (ORCID: https://orcid.org/0009-0007-6080-6136)
- Francesco Crisafi (ORCID: https://orcid.org/0000-0002-6577-7967)
- Moe Vali
- David Pertzborn (ORCID: https://orcid.org/0000-0002-0230-7765)
- Giulio Cerullo (ORCID: https://orcid.org/0000-0002-9534-2702)
- K. K. Mujeeb Rahman
- Píetro Lió (ORCID: https://orcid.org/0000-0002-0540-5053)
- Anna Mühlig (ORCID: https://orcid.org/0009-0001-8278-6494)
- M. Negro (ORCID: https://orcid.org/0000-0002-7317-6598)
- Daniela Pelzel
- Orlando Guntinas‐Lichius (ORCID: https://orcid.org/0000-0001-9671-0784)
- Renzo Vanna (ORCID: https://orcid.org/0000-0001-6218-8393)
- Federico Monti (ORCID: https://orcid.org/0000-0003-3736-891X)
- Andrea Ragni (ORCID: https://orcid.org/0000-0003-4227-570X)
- Gabriele Di Noia
- Tiago Azevedo
- Eleonora Erriquez
- Ulrike Weyer
- Anna Xylander
Institutions
- University of Cambridge (GB)
- Istituto di Fotonica e Nanotecnologie (IT)
- Jena University Hospital (DE)
- Imaging Center (US)
- Politecnico di Milano (IT)
Publication Details
- Published
- 2025-06-23
- DOI
- https://doi.org/10.1109/cleo/europe-eqec65582.2025.11111204
- Primary Topic
- Spectroscopy Techniques in Biomedical and Chemical Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00