Effect of Earthworm (Pheretima posthuma) Extract as Anti-Oxidant, Anti-Inflammatory and Anti-Angiogenesis

Products derived from organisms play an important role in the discovery of new treatments of different chronic and debilitating diseases. Examining the components of annelids has provided a slew of biologically active substances over the years. Antifungal, antibacterial, anti-allergic, antioxidant, anti-inflammatory, and a variety of other properties medically useful properties were found in annelid components. This study investigated the antioxidant, anti-inflammatory, and anti-angiogenic properties of Pheretima posthuma an annelid Asian species. The earthworm's methanol and aqueous extract were tested after it was sacrificed (05 percent ethanol). The anti-angiogenic efficacy of Pheretima posthuma was investigated in-vivo utilizing a Chorio Allantoic Membrane (CAM) assay using distilled water extract., the antioxidant capacity of earthworm (Pheretima posthumous) was determined using methanol extract and in-vitro antioxidant tests of 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and hydrogen per oxide (H₂O₂). A membrane stabilizing assay and a heat induce hemolysis assay utilizing distilled water extract were used to regulate the anti-inflammatory activity of earthworm hypotonicity (HRBCs). Protein denaturation was inhibited by 59.72 percent in the HRBC membrane stabilizing experiment at 80µg/ml. The aqueous extract of Pheretima postmortem exhibits the maximum inhibition at 80µg/ml, i.e., 62.16 percent, in the HRBC membrane stabilizing experiment. The extract was synthesized and tested using an antioxidant DPPH scavenging assay, it produced a great outcome at 40µg/ml, was 54.01 percent. The maximum H₂O₂ scavenging capacity of the Pheretima posthuma methanol extract was recorded 69.80 percent at 80µg/ml. The highest anti-angiogenic activity was detected at 80µg/ml, i.e., 62.16 percent using aqueous extract of Pheretima posthuma. In conclusion, the earthworm extract caused significant changes and has the capacity to influence angiogenesis-growth of new blood vessels; to prevent and treat neoplastic diseases in human beings.