Real-time Dual-functional Monitoring of Temperature and Fluorescence using Flat-field Lens Grating Microspectrometer Integration
An all-optical PCR system integrating a FLG-μSPEC enables real-time temperature sensing and fluorescence-based monitoring of PCR amplification. Utilizing SRB as a temperature-sensitive dye, the system achieved accurate thermal cycling and reliable amplification. Stable performance was demonstrated over 40 cycles, allowing quantitative analysis.
Authors
- Gibeom Kim (ORCID: https://orcid.org/0000-0001-7936-3565)
- Gyuhyeon Han (ORCID: https://orcid.org/0009-0003-5691-3634)
- Ki‐Hun Jeong (ORCID: https://orcid.org/0000-0003-4799-7816)
- Hyejeong Jeong (ORCID: https://orcid.org/0000-0002-7906-1468)
- Jung‐Woo Park (ORCID: https://orcid.org/0000-0002-4019-217X)
Institutions
- Korea Advanced Institute of Science and Technology (KR)
Publication Details
- Published
- 2025-07-13
- DOI
- https://doi.org/10.1109/omn65869.2025.11125931
- Primary Topic
- Analytical Chemistry and Sensors
- Type
- article
- Field-Weighted Citation Impact
- 0.00