Alternating 2D and 3D culture reduces cell size and extends the lifespan of placenta-derived mesenchymal stem cells
The alternating 2D/3D culture system effectively overcomes limitations of conventional MSC expansion by mitigating enlargement, delaying senescence, and preserving both proliferative capacity and immunoregulatory potency. Combined with AlgTube technology, this work demonstrates a promising strategy for MSC manufacturing.
Authors
- Li Han (ORCID: https://orcid.org/0000-0002-2780-0978)
- Yuguo Lei (ORCID: https://orcid.org/0000-0002-7682-6912)
- Wuqiang Zhu (ORCID: https://orcid.org/0000-0001-5217-7789)
- Xinran Wu (ORCID: https://orcid.org/0000-0002-4974-1913)
- Shue Wang (ORCID: https://orcid.org/0000-0001-7382-5378)
- Ying Pan (ORCID: https://orcid.org/0000-0001-9935-6194)
- Liangqi Xie (ORCID: https://orcid.org/0000-0002-6546-9622)
- Aijun Wang (ORCID: https://orcid.org/0000-0002-2985-3627)
- Yakun Yang (ORCID: https://orcid.org/0000-0002-4772-4263)
Institutions
- Pennsylvania State University (US)
- Shriners Hospitals for Children - Erie (US)
- Cleveland Clinic Lerner College of Medicine (US)
- University of New Haven (US)
- Mayo Clinic in Arizona (US)
- University of California Davis Medical Center (US)
- University of California, Davis (US)
Publication Details
- Journal
- Frontiers in Bioengineering and Biotechnology
- Published
- 2025-08-22
- DOI
- https://doi.org/10.3389/fbioe.2025.1632810
- Primary Topic
- Mesenchymal stem cell research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Science Foundation
- National Institutes of Health
- National Heart, Lung, and Blood Institute
- National Cancer Institute
- National Institute of Child Health and Human Development
- Eunice Kennedy Shriver National Institute of Child Health and Human Development