Validation of a plasma GFAP immunoassay and establishment of age-related reference values: bridging analytical performance and routine implementation
This study demonstrates the robust analytical performance of the MSD S-Plex<sup>®</sup> GFAP assay and establishes age-related reference values for plasma GFAP. These findings support its suitability for routine clinical use and enhance its applicability in the diagnosis and monitoring of central nervous system (CNS) pathologies, such as neurodegenerative diseases, neuroinflammatory disorders, and acute brain injuries, within biomarker-supported clinical algorithms.
Authors
- Bjørn‐Eivind Kirsebom (ORCID: https://orcid.org/0000-0002-1413-9578)
- Hlin Kvartsberg (ORCID: https://orcid.org/0000-0002-4481-508X)
- Markus Axelsson (ORCID: https://orcid.org/0000-0003-2036-4007)
- Burak Arslan (ORCID: https://orcid.org/0000-0001-7229-3226)
- Lenka Novakova (ORCID: https://orcid.org/0000-0001-7899-6395)
- Ulf Andréasson (ORCID: https://orcid.org/0000-0001-8622-052X)
- Ingmar Gr. Skoog (ORCID: https://orcid.org/0000-0002-9803-3438)
- Anna Dittrich (ORCID: https://orcid.org/0000-0002-5168-2975)
- Tormod Fladby (ORCID: https://orcid.org/0000-0002-9984-9797)
- Kaj Blennow
- Silke Kern (ORCID: https://orcid.org/0000-0001-7617-7214)
- Henrik Zetterberg (ORCID: https://orcid.org/0000-0002-4671-6763)
- Elzbieta Rembeza
Institutions
- University of Science and Technology of China (CN)
- University Hospital of North Norway (NO)
- University of Wisconsin–Madison (US)
- University of Oslo (NO)
- Akershus University Hospital (NO)
- Sahlgrenska University Hospital (SE)
- Sorbonne Université (FR)
- Institute for Neurodegenerative Disorders (US)
- Institut de Myologie (FR)
- Pitié-Salpêtrière Hospital (FR)
- University of Wisconsin Health (US)
- MRC Prion Unit (GB)
- Institut du Cerveau (FR)
- University College London (GB)
- UiT The Arctic University of Norway (NO)
- University of Gothenburg (SE)
Publication Details
- Journal
- Clinical Chemistry and Laboratory Medicine (CCLM)
- Published
- 2025-08-22
- DOI
- https://doi.org/10.1515/cclm-2025-0870
- Citations
- 1
- Primary Topic
- Cystic Fibrosis Research Advances
- Type
- article
- Field-Weighted Citation Impact
- 1.52
Funders
- Alzheimer's Association
- Amgen
- Alzheimer's Drug Discovery Foundation
- Eli Lilly and Company
- Sanofi
- Biogen
- Teva Pharmaceutical Industries
- Familjen Erling-Perssons Stiftelse
- Cure Alzheimer's Fund
- Stiftelsen för Gamla Tjänarinnor
- EU Joint Programme – Neurodegenerative Disease Research
- Prothena
- Argenx
- Apellis Pharmaceuticals
- European Partnership on Metrology
- National Institute for Health and Care Research
- University College London
- European Commission
- Eisai
- Hjärnfonden
- Novo Nordisk
- Vetenskapsrådet
- Sahlgrenska Universitetssjukhuset
- Göteborgs Universitet
- Siemens Healthineers
- Julius Clinical
- UK Dementia Research Institute
- Olav Thon Stiftelsen
- HORIZON EUROPE Framework Programme