Universal amplification and sequencing of foot-and-mouth disease virus complete genomes using nanopore technology
The amplification, sequencing and bioinformatics strategy developed here has been assessed using a diverse array of FMDV lineages. Using two multiplex PCR reactions, this approach can successfully generate complete genomes of FMDV in a lineage agnostic fashion. Therefore, the primer sets and approaches described here represent a useful tool for expanding the capacity of laboratories to characterise FMDV at the genomic level.
Authors
- Chandana Tennakoon (ORCID: https://orcid.org/0000-0001-8614-6534)
- Andrew E. Shaw (ORCID: https://orcid.org/0000-0001-5761-9769)
- Antonello Di Nardo (ORCID: https://orcid.org/0000-0003-0352-0039)
- Hayley Hicks
- Lina González Gordon (ORCID: https://orcid.org/0000-0002-2063-9771)
- Mark Bronsvoort (ORCID: https://orcid.org/0000-0002-3271-8485)
- Kebaneilwe Lebani (ORCID: https://orcid.org/0000-0001-8927-091X)
- Ugonna E. Ihearahu
- Ryan Waters (ORCID: https://orcid.org/0000-0002-4174-1150)
- Dennis Muhanguzi (ORCID: https://orcid.org/0000-0001-5458-8530)
- Noemi Polo (ORCID: https://orcid.org/0000-0002-0470-2611)
- Donald P. King (ORCID: https://orcid.org/0000-0002-6959-2708)
- Graham Freimanis (ORCID: https://orcid.org/0000-0001-8186-2638)
- Richard J Orton (ORCID: https://orcid.org/0000-0002-3389-4325)
- Nick J. Knowles (ORCID: https://orcid.org/0000-0002-6696-1344)
- Jemma Wadsworth
Institutions
- The Pirbright Institute (GB)
- Roslin Institute (GB)
- Botswana International University of Science and Technology (BW)
- MRC University of Glasgow Centre for Virus Research (GB)
- Makerere University (UG)
Publication Details
- Journal
- BMC Genomics
- Published
- 2025-08-22
- DOI
- https://doi.org/10.1186/s12864-025-11938-7
- Citations
- 2
- Primary Topic
- Animal Disease Management and Epidemiology
- Type
- article
- Field-Weighted Citation Impact
- 3.81
Funders
- Oxford Nanopore Technologies
- UK Research and Innovation
- Department for Environment, Food and Rural Affairs, UK Government
- European Commission
- Directorate for Biological Sciences
- Biotechnology and Biological Sciences Research Council