A Genome-Wide Modeling and Characterization Study of Pleckstrin Homology Domains in Chlamydomonas reinhardtii

The function of pleckstrin homology (PH) domains is to recognize and bind to specific phosphoinositides in the membranes as part of diverse cellular signaling processes. The structure of some PH domains has been solved by X-ray crystallography, but structures of many PH domains remain to be elucidated. In green alga <i>Chlamydomonas reinhardtii</i>, none of the PH domains have been crystallized or characterized. The goal of our study was to model and characterize in detail the structures of all eleven of the PH domains identified in <i>C. reinhardtii</i>. Our computational strategy of integrating the information available on sequence, structure, and function with modeling and biophysical characterization has uncovered new biological predictions for these proteins. These predictions can be validated by future rationally designed experimental studies as an extension of this work. Our results suggest that nine of the eleven <i>C. reinhardtii</i> PH domains show the classical electrostatic polarization of PH domains with a positively charged binding pocket and negatively charged opposing end. Our docking results predict only two PH domains bind specifically to a particular phosphoinositide, while all the other nine PH domains may be able to bind various inositol phospholipids. The lack of preference for a specific phosphoinositide headgroup implies that the positive charge in the binding pocket of the PH domains may be crucial in driving the interaction with the negatively charged phosphoinositides in a non-specific or promiscuous manner. We identified putative homologs of Dynamin GTPase, calcium/calmodulin-dependent kinase, Arf GAP, Rhythm of Chloroplast 23 (ROC23), and oxysterol binding proteins in <i>C. reinhardtii</i> that contain PH domains. In addition, we identified two PH domain-containing proteins that may play a role in the mating process and others that may be important for signaling under phosphate deficiency.