Miniaturized Multicolor Femtosecond Laser Based on Quartz-Encapsulated Nonlinear Frequency Conversion

Ultrafast lasers operating at 740 nm and 820 nm have attracted widespread attention as two-photon light sources for the detection of biological metabolism. Here, we report on a solid-like quartz-encapsulated femtosecond laser with a repetition rate of 80 MHz, delivering 740 nm and 820 nm femtosecond laser pulses. This home-built laser system was realized by employing an erbium-doped 1560 nm fiber laser as the fundamental laser source. A quartz-encapsulated nonlinear frequency conversion stage, consisting of a second-harmonic generation (SHG) stage and self-phase modulation (SPM)-based nonlinear spectral broadening stage, was utilized to deliver 30 mW, 53.7 fs, 740 nm laser pulses and the 15 mW, 60.8 fs, 820 nm laser pulses. Further imaging capabilities of both wavelengths were validated using a custom-built inverted two-photon microscope. Clear imaging results were obtained from mouse kidney sections and pollen samples by collecting the corresponding fluorescence signals. The achieved results demonstrate the great potential of this laser source for advanced two-photon microscopy in metabolic detection.