Generation of four human induced pluripotent stem cell lines from functionally distinct T cell lineages with defined or undefined T cell receptor specificity

T lymphocytes are key contributors to the adaptive immune system. During development, tightly regulated T cell receptor (TCR) gene rearrangement determines antigen specificity and maturation into distinct lineages with pro- or anti-inflammatory functions. From two male donors, we generated four hiPSC lines from isolated T cell lineages (CD4+ conventional helper, CD4+ regulatory, CD8+ cytotoxic). Two lines harbor genetically pre-rearranged TCRs specific to an allogeneic cell line. All lines were generated by integration-free reprogramming using Sendai virus and underwent characterization and quality control. They represent a valuable platform to investigate how a rearranged and pre-selected TCR influences lymphoid differentiation and function.

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Publication Details

Journal
Stem Cell Research
Published
2025-08-22
DOI
https://doi.org/10.1016/j.scr.2025.103813
Primary Topic
CAR-T cell therapy research
Type
article
Field-Weighted Citation Impact
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article

Generation of four human induced pluripotent stem cell lines from functionally distinct T cell lineages with defined or undefined T cell receptor specificity

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Generation of four human induced pluripotent stem cell lines from functionally distinct T cell lineages with defined or undefined T cell receptor specificity

Harald Stachelscheid, Ulrik Stervbo, Julia K. Polansky, Julian Uszkoreit, Valeria Fernández Vallone, Marcel Finke, Christopher Kressler, Michael Schmueck‐Henneresse, Leila Amini, Nina Babel, Kristin Fischer
article en

Abstract

T lymphocytes are key contributors to the adaptive immune system. During development, tightly regulated T cell receptor (TCR) gene rearrangement determines antigen specificity and maturation into distinct lineages with pro- or anti-inflammatory functions. From two male donors, we generated four hiPSC lines from isolated T cell lineages (CD4+ conventional helper, CD4+ regulatory, CD8+ cytotoxic). Two lines harbor genetically pre-rearranged TCRs specific to an allogeneic cell line. All lines were generated by integration-free reprogramming using Sendai virus and underwent characterization and quality control. They represent a valuable platform to investigate how a rearranged and pre-selected TCR influences lymphoid differentiation and function.

Stem Cell ResearchVol. 88
University Hospitals of the Ruhr-University of Bochum (DE), Universitätsklinik Marien Hospital Herne (DE), German Rheumatism Research Centre (DE), Berlin Institute of Health at Charité - Universitätsmedizin Berlin (DE), Ruhr University Bochum (DE)
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CAR-T cell therapy research
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