Widely Targeted Metabolomic and Network Pharmacology Analyses of Active Compounds Enriched from Ethanolic Extract of Oudemansiella raphanipes
<i>Oudemansiella raphanipes</i> ethanolic extract (ORE) was prepared via ultrasonication-assisted ethanolic extraction and enriched through silica gel and macroporous adsorption resin chromatography to afford a non-/weakly polar fraction (ORE-S) and a polar fraction (ORE-N), respectively. This study aimed to (1) quantify major bioactive components (e.g., polyphenols, alkaloids, and terpenes) in ORE-S and ORE-N, (2) assess their antioxidant activities, (3) correlate compositional differences with antioxidant function, and (4) identify key antioxidant compounds along with their potential mechanisms of action. By integrating widely targeted metabolomics with network pharmacology, we not only elucidated how enrichment methods influence the antioxidant properties of ORE but also demonstrated the potential of ORE-N as a valuable source of bioactive compounds and natural antioxidants.
Authors
- Rencai Feng
- Mengxing Chen (ORCID: https://orcid.org/0000-0001-5231-6523)
- Yinfang Wu (ORCID: https://orcid.org/0000-0002-6578-5473)
- Renyun Miao
- Junbin Lin
- Jin Zhao (ORCID: https://orcid.org/0000-0002-6977-8668)
- Bingcheng Gan
- Dan Wu (ORCID: https://orcid.org/0000-0001-5911-5951)
- Xiang Li
- Tao Wang (ORCID: https://orcid.org/0000-0002-0834-4372)
- Shuang Zhu (ORCID: https://orcid.org/0000-0001-6000-1564)
- Zhi Wu (ORCID: https://orcid.org/0000-0001-7097-4837)
Institutions
- Chengdu University (CN)
- Chinese Academy of Agricultural Sciences (CN)
- Sichuan Academy of Agricultural Sciences (CN)
Publication Details
- Journal
- Foods
- Published
- 2025-08-14
- DOI
- https://doi.org/10.3390/foods14162820
- Primary Topic
- Metabolomics and Mass Spectrometry Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Agricultural Science and Technology Innovation Program