Sterilisasi Optimal untuk Kultur Jaringan Bambu Kuning (Bambusa Vulgaris) Secara in Vitro

Yellow bamboo has great potential in various fields, but its conventional propagation has several obstacles. Tissue culture offers a solution to overcome these problems. The focus of this research is the use of appropriate sterilization for in vitro culture. Effective sterilization is a crucial first step in the success of tissue culture. In this study using sterilization in the form of using Nordox 56 WP, without using Nordox 56WP, using Antracol 5 gr solution, and using HgCl2. The parameters observed were the appearance of contaminants in the form of fungi and bacteria and the time of death of the explants. The results showed that the use of HgCl2 can inhibit contaminants and the time of explant death in yellow bamboo plants in vitro.

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Publication Details

Journal
Al-Hayat Natural Sciences Health & Environment Journal
Published
2025-08-22
DOI
https://doi.org/10.47467/alhayat.v3i1.6255
Primary Topic
Plant tissue culture and regeneration
Type
article
Field-Weighted Citation Impact
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article

Sterilisasi Optimal untuk Kultur Jaringan Bambu Kuning (Bambusa Vulgaris) Secara in Vitro

Indriati Husain, Jusna Ahmad, Novri Youla Kandowangko, Miftahul Fikriyah Adudu et al.
Al-Hayat Natural Sciences Health & Environment Journal
Plant tissue culture and regeneration
article

Sterilisasi Optimal untuk Kultur Jaringan Bambu Kuning (Bambusa Vulgaris) Secara in Vitro

Indriati Husain, Jusna Ahmad, Novri Youla Kandowangko, Miftahul Fikriyah Adudu, Devi Bunga Pagalla, Lindawaty Isima
article en

Abstract

Yellow bamboo has great potential in various fields, but its conventional propagation has several obstacles. Tissue culture offers a solution to overcome these problems. The focus of this research is the use of appropriate sterilization for in vitro culture. Effective sterilization is a crucial first step in the success of tissue culture. In this study using sterilization in the form of using Nordox 56 WP, without using Nordox 56WP, using Antracol 5 gr solution, and using HgCl2. The parameters observed were the appearance of contaminants in the form of fungi and bacteria and the time of death of the explants. The results showed that the use of HgCl2 can inhibit contaminants and the time of explant death in yellow bamboo plants in vitro.

Al-Hayat Natural Sciences Health & Environment JournalVol. 3(1)
State University of Gorontalo (ID)
Openalex Percentile: Top 81%
Plant tissue culture and regeneration
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