Ferric reducing antioxidant parameter (FRAP) activity, separation of the flavonoids fraction and folin-ciocalteau assay of Achilleaoligocephala

Background and objective: Acheliaoligocephala DC. is recommended by traditional healers of the Kurdistan Region of Iraq for treating wound healing and gastrointestinal complaints. In medicinal applications, A. oligocephala leaves are crushed and topically placed over the wound. The aim of the study is to investigate the phytochemistry and biochemical properties of the plant. We describe for the first time the flavonoid fraction was separated by preparative thin layer chromatography from the leaves methanolic and 70% methanolic extracts of A. oligocephala and the total poly phenolic content was determined according to the Folin-Ciocalteau assay using gallic acid as the reference standard. Methods: The present study describes the scientific exploration of the antioxidant activity and preparative thin layer chromatography (TLC) separation of the flavonoids fraction and Folin-Ciocalteau Assay of Achelliaoligocephala DC. that has been collected in the Kurdistan Region of Iraq, in which it has been utilized as herbal medicinal remedy. Results: Interestingly, the methanol extracts of leaves showed significant total phenolic contents (70.05%). The flavonoid contents are expressed as mg quercetin equivalent/g of extracts. However, the methanol extracts of leaves exhibited higher flavonoid contents (61.77%). Additionally, the FRAP (Ferric Reducing Antioxidant Parameter) of the obtained extracts were investigated. The results showed values of 6.565 and 14.443 (mol/L Fe2+/g extract), of methanolic and hydroalcoholic leaf extracts respectively. Conclusion: The results obtained in the present study indicated Achelliaoligocephala can acts as a potential source of flavonoids and antioxidant.