DEVELOPMENT OF A SYSTEM OF SUNFLOWER MICROSATELLITE MARKERS FOR MULTIPLEX PCR

Microsatellite DNA-markers are an effective and widespread approach in the passportization system. Using developed by us the multiplex system consisting of seven microsatellite DNAmarkers, it was possible to identify and compile a genetic passport of 47 sunflower lines from V.S. Pustovoit All-Russian Research Institute of Oil Crops in a short time. The markers studied in this work had high discriminatory potential (PIC value above 0.5) and were effective for passportization of genotypes of this collection. According to the results of the compiled genetic passport, the distinguishability of all studied 47 lines by seven microsatellite loci amounted to 100%.