Screening for L1014F mutation inB. rufimanus v1

This protocol describes the process of screening the broad bean bruchid beetle, Bruchus rufimanus (Coleoptera; Chrysomelidae) for the L1014F mutation that has been associated with insecticide resistance in other Coleoptera species (and more widely across insect species). The starting point is a quick and high-throughout non-destructive DNA isolation from beetles, followed by amplification of a region of the Voltage-gated sodium channel gene (VGSC) that harbours the L1014F mutation. The resulting PCR products can be sent for Sanger sequencing to confirm presence/absence of the L1014F mutation.