METAGENOMIC ANALYSIS OF BACTERIA PRODUCING THE ENZYME ADENYLATE CYCLASE (EC 4.6.1.1) UNDER DIFFERENT SOIL AND FOREST MANAGEMENTS

The soil microbial community plays an essential role in ecological processes, such as nutrient cycling and plant health, and is regulated by molecular mechanisms such as signaling via adenylate cyclase (E.C. 4.6.1.1). This enzyme is a key component of the cAMP pathway, with functional relevance in various microbial contexts. The aim of this study was to quantify and taxonomically identify adenylate cyclase-producing bacteria in soils under different agricultural management and in native forest areas in the region of Dourados - MS, Brazil. To this end, five soil samples were analyzed using metagenomic sequencing and bioinformatics tools, including contig assembly, ORF prediction, local database alignment, taxonomic and statistical analyses. 18,139 sequences associated with the target enzyme were identified, with greater abundance in soils of continuous pasture and crop-livestock integration. The phyla Actinobacteria and Proteobacteria were predominant, and the genera Sinorhizobium, Micromonospora and Bifidobacterium stood out, with significant variations between managements. The analysis showed that management practices directly affect the composition and diversity of bacteria producing the enzyme adenylate cyclase.