Whole-Genome DNA Extractions for MinION Sequencing Using the Macherey-Nagel NucleoBond HMW DNA Kit v1

Long-read sequencing is increasingly accessible, enabling unprecedented insights into the pervasive structural rearrangements of fungal genomes. In this study, we optimized a genomic DNA extraction protocol to obtain unsheared, high-molecular-weight DNA suitable for both PacBio and Oxford Nanopore Technologies sequencing. We validated the protocol with both sequencing platforms, achieving in both case telomere-to-telomere genome assembly of natural isolates of the fungal ascomycete Zymoseptoria tritici.