Figure 5 from Gut Microbiome Profiling in Eμ-TCL1 Mice Reveals Intestinal Changes and a Dysbiotic Signature Specific to Chronic Lymphocytic Leukemia

<p>Dysbiotic microbiome is observed in the aggressive syngeneic model of CLL. <b>A,</b> Study schematic illustrating WT B6 mice were engrafted intravenously via the tail vein with 1e<sup>7</sup> spleen-derived lymphocytes from a moribund Eμ-TCL1 mouse (CLL engraftment). Engrafted mice (<i>n</i> = 10) were monitored with serial blood and fecal pellet sample collections over the study course of 7 weeks. <b>B,</b> CLL disease burden was measured by the percentage of CD45<sup>+</sup>/CD19<sup>+</sup>/CD5<sup>+</sup> cells in the peripheral blood via flow cytometric analysis starting 1 week after engraftment. Asterisks denote the significance between leukemic mice over time (***, <i>P</i> < 0.001). One-way ANOVA with Dunnett multiple comparisons was applied for testing. <b>C,</b> Alpha diversity measured by the Shannon index in leukemic mice over 7 weeks. Data are presented as box-and-whisker plots, with box edges representing the 25th and 75th percentiles, the center line showing the median value, and whiskers extending to minimum and maximum values. One-way ANOVA with Dunnett multiple comparisons was applied for testing. <b>D,</b> PCoA plot depicting Bray–Curis distance as a measure of beta diversity in leukemic mice over 7 weeks. <b>E,</b> Relative abundance bar plots of bacterial taxa present in the gut microbiome of diseased mice over 7 weeks following engraftment. Unassigned includes all remaining taxa present in the microbiome at decreased abundance. <b>F,</b> LEfSe analysis demonstrating enrichment of specific bacterial taxa at the phylum level in leukemic mice at the time of engraftment and 1 and 3 weeks after engraftment. Eng./engraft., engraftment. LDA, linear discriminant analysis.</p>