Supplementary Figure S3 from Development of an Anti-CD99 Antibody Enables Targeting of Diffuse Midline Glioma

<p>Validation of depletion of CD99 in DMG cells by Western blotting and flow cytometry. Evaluation of functional effects of CD99-depleted cells, using cell proliferation assays, stemness by Aldefluor assay, differentiation by qRT-PCR, in vivo tumor growth inhibition, and apoptotic and differentiation markers.</p>

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Publication Details

Published
2025-08-15
DOI
https://doi.org/10.1158/0008-5472.29918861
Primary Topic
Glioma Diagnosis and Treatment
Type
article
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article

Supplementary Figure S3 from Development of an Anti-CD99 Antibody Enables Targeting of Diffuse Midline Glioma

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Supplementary Figure S3 from Development of an Anti-CD99 Antibody Enables Targeting of Diffuse Midline Glioma

Rajeev Vibhakar, Breauna Brunt, Bridget Sanford, Ammu Suresh, Krishna Madhavan, Senthilnath Lakshmanachetty, Ilango Balakrishnan, Sujatha Venkataraman, Adam L. Green, Etienne Danis, Angela Pierce, Siddhartha S. Mitra, Nathan Davidson, Nathan Dahl, John DeSisto, Faye Walker, Zachary James Nuss, Andrew M. Donson, Joshua Michlin, Dong Wang, Kenneth L. Jones
article en

Abstract

<p>Validation of depletion of CD99 in DMG cells by Western blotting and flow cytometry. Evaluation of functional effects of CD99-depleted cells, using cell proliferation assays, stemness by Aldefluor assay, differentiation by qRT-PCR, in vivo tumor growth inhibition, and apoptotic and differentiation markers.</p>

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Glioma Diagnosis and Treatment
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