Figure S3 from Reactivating cGAS–STING Signaling by Targeting SOS1 Enhances Antitumor Immunity in <i>NRAS</i>-Mutant Tumors
<p>Supplementary Fig. S3. The suppression of cGAS-STING pathway in <i>NRAS</i> mutant cells mediates immune escape. A. Flow cytometry measuring the DCs mature markers (MHC II and CD86), when co-cultured with the indicated tumor cells pre-treated with poly(dA:dT). B. Immunoblot in NCI-H1299 cells expressing sgRNA targeting STING. C. Flow cytometry measuring the DCs mature markers (MHC II and CD86), when co-cultured with the indicated tumor cells pre-treated with HT-DNA. D-E. Flow cytometry measuring the cytotoxicity of PBMCs that killing the cancer cells pre-treated with poly(dA:dT) (D) and HT-DNA(E). F. FACS analysis of CD8<sup>+</sup>T, NK1.1<sup>+</sup>, and IFNγ<sup>+</sup> or Granzyme B<sup>+</sup> in CD8<sup>+</sup> T cell populations and NK cell populations from the isolated TILs. Representative dot plots from a representative mouse for each group.</p>
Authors
- Xiao‐Feng Zhu (ORCID: https://orcid.org/0000-0002-1250-0796)
- Tian Du (ORCID: https://orcid.org/0000-0003-0841-6484)
- Kai-Ming Zhang (ORCID: https://orcid.org/0000-0002-8195-1136)
- Yu-Hong Chen
- Hai‐Liang Zhang (ORCID: https://orcid.org/0009-0006-0098-7580)
- Rong Deng (ORCID: https://orcid.org/0000-0003-3724-0326)
- Jia‐Lu Shan
- Zhi‐Ling Li
- Wen-Qing Zhong
- Yun Huang (ORCID: https://orcid.org/0000-0001-6963-3728)
- Jia‐Hong Tang
- Dong Yang (ORCID: https://orcid.org/0000-0002-4358-9631)
- Xiao-Yu Yang
Publication Details
- Published
- 2025-08-15
- DOI
- https://doi.org/10.1158/0008-5472.29918738
- Primary Topic
- interferon and immune responses
- Type
- article
- Field-Weighted Citation Impact
- 0.00