Tree Age-Related Differences in Chilling Resistance and Bark-Bleeding Physiological Responses to Chemical Component and Fiber Morphology Changes in Cell Walls of Hevea brasiliensis Bark

The purpose of this study was to establish the relationship between the chilling resistance of rubber trees and the bark-bleeding characteristics caused by chilling stress, considering physiological indicators in rubber tree bark, cell wall chemical components, fiber morphologies, and tensile properties. This offered a unique perspective for examining the underlying mechanisms of latex bleeding and chilling stress in <i>Hevea brasiliensis</i>. One-year-old seedlings and two-year-old twig segments in five- and twenty-one-year-old rubber trees (5YB and 21YB) were used to compare the age-mediation differences in their various parameters. Meanwhile, the LT₅₀ values were calculated with Logistic regression analysis of relative electrical conductivity (REC) data under gradient low temperatures. Subsequently, changes in corresponding parameters of 1-year-old seedling stem bark at different ages were determined, and the bark-bleeding characteristics of seedlings and twig segments were analyzed under artificially simulated chilling stress, respectively. A correlation analysis between semi-lethal temperature (LT₅₀) values, relative water content (RWC) values, bark-bleeding characteristics, cell-wall chemical component contents, fiber dimensions, and tensile property parameters was implemented to estimate interrelationships among them. The LT₅₀ values ranged from -2.0387 °C to -0.8695 °C. The results showed that the chilling resistance order of rubber trees at different ages was as follows: 21YB (2-year-old twig bark from 21-year-old rubber trees) > 5YB (2-year-old twig bark from 5-year-old rubber trees) > SLB (semi-lignification bark in 1-year-old seedlings) > GB (green bark in 1-year-old seedlings). The chilling resistance of seedlings and twig segments in rubber trees was highly positively (<i>p</i> < 0.001) related to fiber morphologies. Chilling-induced bark-bleeding characteristics were significantly correlated (<i>p</i> < 0.001) with fiber morphologies, bark tensile properties, and cell-wall components. The analysis data in this study contribute towards building a comprehensive understanding of the mechanisms of chilling-induced bark bleeding needed not only in rubber tree cultivation but also in sustainable rubber production.