Evaluation of the HumanMethylationEPIC v2.0 Bead Chip Using Low Quality and Quantity DNA Samples
Both DNA fragment size and DNA input amounts affect DNAm analysis on the EPIC v2.0, with the investigated DNA fragment size having a greater impact than the investigated DNA input amount. DNAm measurements were achieved with the EPIC v2.0 microarray down to an average DNA fragment size of 165 bp and a 20 ng DNA input. Highly fragmented DNA (95 bp) did not result in usable DNAm analysis as all samples failed QC. Overall, our study demonstrates the potential and limitations of EPIC v2.0 microarray with low quality and quantity DNA samples.
Authors
- Athina Vidaki (ORCID: https://orcid.org/0000-0002-5470-245X)
- Mikkel Eriksen Dupont (ORCID: https://orcid.org/0000-0002-3544-7393)
- Brando Poggiali
- Marie‐Louise Kampmann (ORCID: https://orcid.org/0000-0001-5403-5960)
- Jeppe Dyrberg Andersen (ORCID: https://orcid.org/0000-0002-4395-5232)
- Claus Børsting (ORCID: https://orcid.org/0000-0003-0421-7429)
- Vânia Pereira (ORCID: https://orcid.org/0000-0002-8789-5286)
- Jacob Tfelt‐Hansen (ORCID: https://orcid.org/0000-0003-3895-9316)
Institutions
- University of Copenhagen (DK)
- Copenhagen University Hospital (DK)
- Rigshospitalet (DK)
- Maastricht University (NL)
Publication Details
- Journal
- Biological Procedures Online
- Published
- 2025-08-21
- DOI
- https://doi.org/10.1186/s12575-025-00292-3
- Citations
- 4
- Primary Topic
- Epigenetics and DNA Methylation
- Type
- article
- Field-Weighted Citation Impact
- 3.22
Funders
- Københavns Universitet