Generation of human embryonic stem cell line expressing dCas9-TET1 fusion protein for epigenetic editing
CRISPR-based epigenome editing systems can induce site-specific transcriptional activation or repression of target genes. Ten-eleven translocation methylcytosine dioxygenase 1 (TET1) is a transcriptional activation effector involved in the cytosine demethylation of CpG dinucleotides in gene regulatory regions. In this study, we generated a human embryonic stem cell line that stably expresses catalytically dead Cas9 (dCas9) fused to the catalytic domain of TET1 via lentiviral transduction. This cell line can be used for locus-specific transcriptional activation in combination with single guide RNAs and serves as a valuable tool for epigenetic regulation in stem cell and organoid models.
Authors
- Eunhye Kang
- Ji Hyeon Ryu (ORCID: https://orcid.org/0000-0003-0342-3108)
- Seongyea Jo
- Haneul Noh
- Han‐Jin Park (ORCID: https://orcid.org/0000-0002-0113-4145)
- Ji‐Woo Kim (ORCID: https://orcid.org/0000-0002-4070-3021)
- Hyemin Kim (ORCID: https://orcid.org/0000-0002-3440-8456)
Institutions
- Korea Institute of Toxicology (KR)
Publication Details
- Journal
- Stem Cell Research
- Published
- 2025-08-21
- DOI
- https://doi.org/10.1016/j.scr.2025.103811
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Korea Institute of Toxicology
- National Research Foundation of Korea
- Ministry of Science and ICT, South Korea