Identifying new targets for improving terpenoid biosynthesis in Yarrowia lipolytica through random genomic cytosine base editing
Helicase-CDA system circumvents the dependency on NHEJ-mediated whole-genome mutation approach, offering a robust tool for continuous genome evolution in pre-engineered industrial strains. This study not only enhances genome editing in Y. lipolytica but also identifies a practical target for optimizing terpenoid biosynthesis, demonstrating significant potential for applications in metabolic engineering and synthetic biology.
Authors
- QingYan Li
- Lei Zhu (ORCID: https://orcid.org/0000-0003-1685-7968)
- Zhe Sun (ORCID: https://orcid.org/0000-0002-8258-7946)
- Zhenxia Li (ORCID: https://orcid.org/0000-0003-3216-781X)
- Xueli Zhang (ORCID: https://orcid.org/0000-0002-8660-4037)
- Rongtao Lv
Institutions
- Tianjin University of Science and Technology (CN)
- Tianjin Institute of Industrial Biotechnology (CN)
- Henan Institute of Science and Technology (CN)
Publication Details
- Journal
- Microbial Cell Factories
- Published
- 2025-08-20
- DOI
- https://doi.org/10.1186/s12934-025-02819-5
- Citations
- 3
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 2.42
Funders
- National Natural Science Foundation of China