Constructing the optimal experimental autoimmune thyroiditis mouse model using porcine thyroglobulin
The combination of high-dose pTg (200 μg) and three immunizations maximally induced AIT pathology and immune responses in NOD/LtJ mice. Tail vein injection excelled in stimulating antibody production and NLRP3 activation, while subcutaneous injection promoted stronger histological inflammation. After balancing operational feasibility, pathological reproducibility, and immunological specificity, three subcutaneous and intravenous tail injection of 200 μg pTg are recommended as the optimal modeling protocol. This approach accelerates model selection, improves experimental efficiency, and reduces animal use, providing a robust foundation for AIT research.
Authors
- Xiangkun Meng (ORCID: https://orcid.org/0000-0001-8830-9552)
- Jinli Luo (ORCID: https://orcid.org/0000-0001-8077-2948)
- Xiaotong Yu (ORCID: https://orcid.org/0000-0002-8184-5181)
- Lin Han (ORCID: https://orcid.org/0000-0001-9725-1946)
- Ke Liu (ORCID: https://orcid.org/0009-0005-5113-2112)
- Pei Zhang (ORCID: https://orcid.org/0000-0001-9621-107X)
- Zishan Jin
Institutions
- Beijing University of Chinese Medicine (CN)
- Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
- Ji Hua Laboratory (CN)
- Fosun Pharma (China) (CN)
- Changchun University of Chinese Medicine (CN)
- Donlim (China) (CN)
- Guang’anmen Hospital (CN)
Publication Details
- Journal
- Frontiers in Immunology
- Published
- 2025-08-20
- DOI
- https://doi.org/10.3389/fimmu.2025.1591196
- Citations
- 1
- Primary Topic
- Immune responses and vaccinations
- Type
- article
- Field-Weighted Citation Impact
- 0.80
Funders
- China Academy of Chinese Medical Sciences
- China Academy of Traditional Chinese Medicine