Temporal trends of a cellular host response test for sepsis and a comparison with selected biomarkers of inflammation and infection

Sepsis, a condition characterized by immune dysregulation, is the leading cause of in-hospital mortality and requires rapid treatment. Assessing immune dysregulation has been challenging. The IntelliSep Index (ISI), a novel biomarker which leverages microfluidic deformability cytometry to assess immune activation, has been evaluated as a test for Emergency Department use but it is unclear how the signal evolves as patient condition evolves. In a 47-patient cohort with hospital stays ≤ 14 days, ISI provided a good indicator of disease progression by blinded physician review. ISI trends correlated with clinical improvement over time. During the first 12 h following presentation, ISI was unaffected by antibiotic treatment initiated in the emergency department (ED). After this period however, ISI values declined, reflecting the patient's response to treatment, with Band 3 patients showing a 20% decline within the first two days of admission. ISI trends preceded Sequential Organ Failure Assessment (SOFA) score changes by two days. ISI also provided more stable and timely disease state assessments compared to traditional biomarkers (PCT, CRP, IL-6, and neutrophil elastase). Larger studies are needed to validate these findings and assess ISI's clinical utility as a prognosticator of sepsis progression and treatment response.

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Publication Details

Journal
Scientific Reports
Published
2025-08-20
DOI
https://doi.org/10.1038/s41598-025-14860-w
Primary Topic
Sepsis Diagnosis and Treatment
Type
article
Field-Weighted Citation Impact
0.00
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article

Temporal trends of a cellular host response test for sepsis and a comparison with selected biomarkers of inflammation and infection

Katherine Fenstermacher, Guillaume Spielmann, Richard E. Rothman, Matthew S. Berlinger et al.
2 links
Scientific Reports
Sepsis Diagnosis and Treatment
article

Temporal trends of a cellular host response test for sepsis and a comparison with selected biomarkers of inflammation and infection

Katherine Fenstermacher, Guillaume Spielmann, Richard E. Rothman, Matthew S. Berlinger, Camille King, David N. Hager, D. I. Robertson, Heather Quiriarte, Roya Sheybani, Tonya Jagneaux, Matthew G. Sorrells, Susan Park Ochsner, Henry T. K. Tse, Hollis R. O’Neal, Robert B. Scoggins, Ajay M. Shah, Gideon D. Avornu, Elijah James Kurien, Christopher B. Thomas, Youyoung Kim, James Walker, J. Gavin Daigle, Adenine Cembellin-Kao, Chase Yonamine, Querino Maia, Maciej Obrebski
article en

Abstract

Sepsis, a condition characterized by immune dysregulation, is the leading cause of in-hospital mortality and requires rapid treatment. Assessing immune dysregulation has been challenging. The IntelliSep Index (ISI), a novel biomarker which leverages microfluidic deformability cytometry to assess immune activation, has been evaluated as a test for Emergency Department use but it is unclear how the signal evolves as patient condition evolves. In a 47-patient cohort with hospital stays ≤ 14 days, ISI provided a good indicator of disease progression by blinded physician review. ISI trends correlated with clinical improvement over time. During the first 12 h following presentation, ISI was unaffected by antibiotic treatment initiated in the emergency department (ED). After this period however, ISI values declined, reflecting the patient's response to treatment, with Band 3 patients showing a 20% decline within the first two days of admission. ISI trends preceded Sequential Organ Failure Assessment (SOFA) score changes by two days. ISI also provided more stable and timely disease state assessments compared to traditional biomarkers (PCT, CRP, IL-6, and neutrophil elastase). Larger studies are needed to validate these findings and assess ISI's clinical utility as a prognosticator of sepsis progression and treatment response.

Scientific ReportsVol. 15(1)
Louisiana State University (US), Our Lady of the Lake Regional Medical Center (US), Johns Hopkins University (US), Cytovale (United States) (US), Kootenai Medical Center (US), Louisiana State University Health Sciences Center New Orleans (US), Franciscan Missionaries of Our Lady University (US)
Openalex Percentile: Top 70%
Sepsis Diagnosis and Treatment
DOIOpen Access PDFOpenAlex
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