AN OPTIMIZED KINETIC SPECTROPHOTOMETRIC METHOD FOR THE RAPID AND ACCURATE DETERMINATION OF CEFOPERAZONE IN URINE AND TAP WATER SAMPLES

This study presents a new approach for quantifying the antibiotic cefoperazone (CPZ) in spiked urine and tap water samples using an indirect kinetic spectrophotometry method.The method involves the oxidation of CPZ by permanganate (MnO4 -) in an alkaline media.The progress of the reaction is monitored by tracking the increase in manganate (MnO4 2-) at 610 nm.The optimization of chemical-dependent variables was achieved through the utilization of multivariate statistical methods.The optimized values were 5 minute of reaction time, KMnO4 of 1.11 10 -3 mol L -1 , and NaOH of 0.27 mol L -1 .Under these conditions, calibration curves were constructed.The detection limits obtained in urine and tap water spiked samples were 3.75 10 -7 mol L -1 and 3.74 10 -7 mol L -1 , respectively.The results showed that intraday and interday recoveries ranged from 98.25 to 102.7%, indicating acceptable differences between observed and expected values according to the confidence percentage established as a criterion of acceptability; thus, demonstrating repeatability in results as well as satisfactory accuracy for this kinetic analytical method.