Converging Patterns of Heterotrophic Respiration Between Growing and Non-Growing Seasons in Northern Temperate Grasslands

Temperate grasslands are highly sensitive to climate change and play a crucial role in terrestrial carbon cycling. In the context of global warming, heterotrophic respiration (Rh) has intensified, contributing significantly to atmospheric CO₂ emissions. However, seasonal patterns of Rh, particularly differences between the growing season (GS) and non-growing season (non-GS), remain poorly quantified. This study used daily eddy covariance data from multiple flux towers combined with MODIS GPP and NPP products to estimate Rh across temperate grasslands from 2002 to 2021. We examined interannual variations in GS and non-GS Rh contributions and assessed their relationships with key hydrothermal variables. The results showed that mean Rh during GS and non-GS was 527 ± 357 and 341 ± 180 g C m^-2 yr^-1, respectively, accounting for 57.8 ± 14.6% and 42.2 ± 14.6% of the annual Rh. Moreover, GS Rh exhibited a declining trend, while non-GS Rh increased over time, indicating a gradual convergence in their seasonal contributions. This pattern was primarily driven by increasing drought stress in GS and warmer, moderately moist conditions in non-GS that favored microbial activity. Our findings underscore the necessity of distinguishing seasonal Rh dynamics when investigating global carbon cycle dynamics. Future earth system models should place greater emphasis on seasonal differences in soil respiration processes by explicitly incorporating the influence of soil moisture on the decomposition rate of soil organic carbon, in order to improve the accuracy of carbon release risk assessments under global change scenarios.