prime-seq 2 v1

This is the optimized version of the prime-seq protocol. We systematically optimized the powerful and cost-efficient bulk RNA-seq protocol prime-seq. Notable efficiency enhancements were achieved by addressing DNase digestion, reverse transcription, adapter ligation, and amplification. The optimized protocol increased final unique molecular identifiers (UMIs) by 60 % at equal sequencing costs or, conversely, reduced sequencing costs by 38 % at equal counts. This further improves one of the most cost-efficient bulk RNA-seq protocols available, making it currently one of the best bulk RNA-seq options.