Transcriptomic and Proteomic Insights into 6PPD/6PPD-Q Induced Oxidative Stress in Black-Spotted Frogs

N-(1,3-dimethylbutyl)-N'-phenyl-p-phenylenediamine (6PPD) and its oxidation product 6PPD-quinone (6PPD-Q) can have lethal effects on aquatic organisms, interfering with gene expression and protein content in aquatic animals. In this study, we performed proteomics and transcriptomics analyses on the livers of black-spotted frogs exposed to 6PPD and 6PPD-Q. The results showed that 6PPD and 6PPD-Q can cause oxidative stress in the liver, significantly reducing catalase (CAT) and glutathione peroxidase (GSH-Px) levels, with 6PPD-Q having a more significant toxic effect. Through transcriptomics and proteomics analysis, this study identified oxidative stress and immune defense pathways. In this study, the liver of the black-spotted frog provided some molecular insights into the toxicity of 6PPD and 6PPD-Q. Nonetheless, additional investigations are required to gain a clearer comprehension of the possible mechanisms that drive how aquatic organisms react to the toxic effects of 6PPD and 6PPD-Q.