Collagenase Production from Aspergillus serratalhadensis URM 7866 Using Industrial By-Products: Purification and Characterization
Collagenases are enzymes with broad biotechnological applications in medicine. This study describes the production and characterization of a collagenase from Aspergillus serratalhadensis URM 7866, isolated from the Caatinga biome. Solid-state fermentations were conducted using wheat bran under varying conditions of pH (6, 7, 8), moisture content (50%, 60%, 70%), and substrate concentration (2.5 g, 5 g, 10 g). The optimal condition—10 g of wheat bran at pH 8 and 70% moisture—yielded the highest collagenolytic activity (177.96 U/mL) and a specific activity of 50.55 U/mg. The enzyme was purified via multiple chromatography, with pre-purification and final purification factors of 18.09 and 20.21, respectively, reaching a specific activity of 1021.86 U/mg. The enzyme showed optimal activity at 50 °C and pH 8, with stability from 20 to 40 °C and pH 7–9. PMSF caused >80% inhibition; EDTA caused ~34% inhibition. Activity increased with Na+ and Ca2+ and was inhibited by Zn2+. The enzyme retained full activity in anionic and non-ionic surfactants (1–10%). FTIR confirmed characteristic amide bands, and kinetic analysis revealed a Km of 1.72 mg/mL and Vmax of 6.89 mg/mL/min. These findings support its potential for alkaline and surfactant-rich industrial processes.
Authors
- Daniel Charles dos Santos Macêdo (ORCID: https://orcid.org/0000-0002-2356-5149)
- Daniela de Araújo Viana Marques (ORCID: https://orcid.org/0000-0002-2380-7910)
- Ana Lúcia Figueiredo Porto (ORCID: https://orcid.org/0000-0001-5561-5158)
- Raphael Luiz Andrade Silva (ORCID: https://orcid.org/0000-0001-9039-5293)
- Pietra Gícia Oliveira Cosmo da Silva
- Márcia Nieves Carneiro da Cunha (ORCID: https://orcid.org/0000-0003-2744-6216)
- Thiago Pajeú Nascimento (ORCID: https://orcid.org/0000-0003-3480-6734)
- Romero Marcos Pedrosa Brandão Costa (ORCID: https://orcid.org/0000-0001-7045-2975)
- Luiz Henrique Svintiskas Lino
- Kethylen Bárbara Barbosa Cardoso (ORCID: https://orcid.org/0000-0003-0317-0307)
- Cristina Maria de Souza‐Motta (ORCID: https://orcid.org/0000-0002-0964-8271)
- Maria Eduarda Luiz Coêlho de Miranda (ORCID: https://orcid.org/0000-0002-7102-2800)
- Carolina Duarte (ORCID: https://orcid.org/0000-0001-7596-8292)
Institutions
- Universidade Federal de Pernambuco (BR)
- Universidade Federal do Piauí (BR)
- Universidade Federal Rural de Pernambuco (BR)
- Universidade de Pernambuco (BR)
Publication Details
- Journal
- Fermentation
- Published
- 2025-08-20
- DOI
- https://doi.org/10.3390/fermentation11080478
- Citations
- 1
- Primary Topic
- Enzyme Production and Characterization
- Type
- article
- Field-Weighted Citation Impact
- 1.23
Funders
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
- Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco