The effect of rotenone contamination on high-resolution mitochondrial respiration experiments
Our findings highlight an important methodological consideration regarding the measurement of mitochondrial respiratory function using high-resolution respirometry, with inhibitor contamination potentially affecting the conclusions derived from experiments conducted in close succession. Future studies investigating mitochondrial respiratory function should assess the necessity of using inhibitors such as rotenone, ensure thorough wash procedures between experiments, and explicitly report the washing protocols used.
Authors
- Najaf Zare (ORCID: https://orcid.org/0000-0002-0338-0622)
- Dale F. Taylor (ORCID: https://orcid.org/0000-0001-6766-6948)
- Nicholas J. Saner (ORCID: https://orcid.org/0000-0002-6011-7126)
- Hanzhe Li
- Elizabeth G. Reisman (ORCID: https://orcid.org/0000-0002-5791-3324)
- David John Bishop (ORCID: https://orcid.org/0000-0002-6956-9188)
- Xu Yan (ORCID: https://orcid.org/0000-0001-8547-4210)
- Jia Li (ORCID: https://orcid.org/0000-0001-7132-2574)
- Andrew Garnham (ORCID: https://orcid.org/0000-0001-8068-9300)
- Jujiao Kuang (ORCID: https://orcid.org/0000-0002-1366-0089)
- Matthew J-C Lee (ORCID: https://orcid.org/0000-0001-9937-3445)
- Jia Wei (ORCID: https://orcid.org/0000-0003-1125-1377)
Institutions
- Deakin University (AU)
- Victoria University (AU)
- Australian Catholic University (AU)
Publication Details
- Journal
- PeerJ
- Published
- 2025-08-14
- DOI
- https://doi.org/10.7717/peerj.19879
- Primary Topic
- Metabolomics and Mass Spectrometry Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.00