Molecular-biological diagnostic as strategy for quality control and genetic purity of benefi cial insects Trichogramma spp.

The study focuses on the development of molecular diagnostic methods for verifying the genetic purity of Trichogramma spp. entomophagous insect cultures used in biological pest control. Analysis of the internal transcribed spacer ITS2 of ribosomal DNA revealed interspecific differences and identified mixed populations. Polymerase chain reaction, sequencing, and nucleotide sequence comparison ensured accurate determination of genetic consistency. The results demonstrated impurities in Trichogramma pintoi and T. evanescens samples, whereas T. brassicae and T. ahea confirmed purity. To maintain the effectiveness of biological control, regular monitoring of genetic purity is recommended every three months after two generations. Implementing these methods reduces chemical pesticide reliance, promoting ecosystem stability and organic product quality.