Spheroid Cell Aggregation Enhanced by Enzyme‐Free Ultrasound‐Detached Cells (Adv. Biology 8/2025)
Spheroid Cell Aggregation In article number 2500092, Kenjiro Takemura and co-workers demonstrate that ultrasound-based, enzyme-free cell detachment accelerates spheroid formation by preserving surface proteins, reducing variability, and enhancing cell aggregation. Comparable engraftment and improved spatial organization in co-cultured spheroids were observed. These findings suggest ultrasound detachment as a robust alternative for efficient, high-quality spheroid production in tissue engineering and related biomedical applications.
Authors
- Jun Homma (ORCID: https://orcid.org/0009-0001-1976-0670)
- Makoto Hirano (ORCID: https://orcid.org/0000-0001-5806-8639)
- Chikahiro Imashiro (ORCID: https://orcid.org/0000-0002-0716-2585)
- Julien van Delft
- Yuta Kurashina (ORCID: https://orcid.org/0000-0003-3752-7204)
- Kenjiro Takemura (ORCID: https://orcid.org/0000-0002-0298-5558)
- Shinsuke MOCHIZUKI
- Hideharu Shimozawa
Institutions
- Canon (Japan) (JP)
- Tokyo University of Technology (JP)
- Keio University (JP)
- Tokyo Women's Medical University (JP)
- Yasuda Women's University (JP)
- Tokyo University of Agriculture and Technology (JP)
Publication Details
- Journal
- Advanced Biology
- Published
- 2025-08-01
- DOI
- https://doi.org/10.1002/adbi.70039
- Primary Topic
- 3D Printing in Biomedical Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00