Impact of blended used cooking oil-kitchen waste on the bio-recycler, black soldier Fly larvae

Abstract As populations grow, the quantity of organic waste increases dramatically. This organic waste includes agricultural waste, kitchen waste, and used cooking oil (UCO). The improper management of organic waste leads to serious environmental concerns. This study investigates the biochemical impact of mixing kitchen waste with UCO mixing on Black Soldier Fly (BSF) larvae, is reflected as promising implement for the recycling process of organic waste. The paper evaluated oxidative stress responses in 5 th instar BSF larvae reared on control food, kitchen waste (KW), and KW mixed with UCO. Lipid peroxidation, superoxide anion radical (O 2 • – ), superoxide dismutase (SOD) activity, and antiradical activity (DPPH) were measured in larval guts to evaluate the consequence of UCO exposure on the larval oxidative stress system. The outcomes revealed that the elevated levels of reactive oxygen species (ROS), oxidative stress damage (OSD), and antioxidant enzymes and compounds in the treated groups (KW and KW+UCO) compared to other experimental treatments. This research contributes to understanding the potential risks of UCO mixing within BSF-based organic waste management systems therefore pushing the development of sustainable waste treatment strategies.