Optimization of a Western blot protocol for the detection of low levels of tissue factor in human cells
Researchers should consider each step in Western blotting when establishing a method for detecting low-abundance antigens, such as TF.
Authors
- Ana Teresa Azevedo Sachetto (ORCID: https://orcid.org/0000-0002-8234-1209)
- Michael V. Perkins (ORCID: https://orcid.org/0000-0003-1706-2315)
- Nigel Mackman (ORCID: https://orcid.org/0000-0002-9170-7700)
Institutions
- University of North Carolina at Chapel Hill (US)
Publication Details
- Journal
- Research and Practice in Thrombosis and Haemostasis
- Published
- 2025-08-01
- DOI
- https://doi.org/10.1016/j.rpth.2025.103016
- Citations
- 1
- Primary Topic
- Tissue Engineering and Regenerative Medicine
- Type
- article
- Field-Weighted Citation Impact
- 2.01
Funders
- American Heart Association
- National Institutes of Health
- National Heart, Lung, and Blood Institute