Quantitative Nano-Artifacts Exposure Toward Single Cells Utilizing a Microdroplet-Based Microfluidic System
We propose a microfluidic system that enables quantitative nano-artifacts exposure to single cells. Unlike a conventional cell analysis based on pipetting, our system allows precise control of the desired concentration and the time periods, particularly the start timing of the exposure, by encapsulating suspensions containing nano-artifacts and single cells. In this presentation, we evaluate the basic performance of the constructed system. Moreover, we demonstrate an unprecedented cell analysis application: “monitoring of cell death using engineered cells”. The proposed method would open new paradigms for analyses of individual cellular responses.
Authors
- Niko Kimura (ORCID: https://orcid.org/0000-0002-6313-604X)
- Takashi Kamatani (ORCID: https://orcid.org/0000-0001-7164-0513)
- Yoshitaka Shirasaki (ORCID: https://orcid.org/0000-0002-3808-1630)
- Makoto Saito (ORCID: https://orcid.org/0000-0002-3512-4412)
- Shigeo S. Sugano (ORCID: https://orcid.org/0000-0001-9986-2839)
- Ren Takeuchi
- Chia-Hung Tsai (ORCID: https://orcid.org/0000-0002-9792-1169)
- Shinya Sakuma (ORCID: https://orcid.org/0000-0002-4145-7264)
- Kosuke Dodo (ORCID: https://orcid.org/0000-0001-6008-2915)
- Yoko Yamanishi (ORCID: https://orcid.org/0000-0001-7193-9339)
- Nariaki Kiyama
- Satoshi Yotsumoto
- Rinko Kurogi
Institutions
- Kyushu Kyoritsu University (JP)
- Kyushu University (JP)
- National Yang Ming Chiao Tung University (TW)
- Tokyo University of Pharmacy and Life Sciences (JP)
- Shanghai Institute for Science of Science (CN)
- RIKEN Center for Advanced Intelligence Project (JP)
- Eiken Chemical (Japan) (JP)
- Nitto RIKEN (Japan) (JP)
- National Institute of Advanced Industrial Science and Technology (JP)
- The University of Tokyo (JP)
- Tokyo University of Agriculture and Technology (JP)
Publication Details
- Published
- 2025-06-29
- DOI
- https://doi.org/10.1109/transducers61432.2025.11110425
- Primary Topic
- Microfluidic and Bio-sensing Technologies
- Type
- article
- Field-Weighted Citation Impact
- 0.00