Reactive Oxygen Species Generation in Microfluidics Device by Plasma Excitation via Thin Film

We achieved reactive oxygen species (ROS) generation in a closed microchannel by plasma excitation via thin dielectric film. Surprisingly, short-lived ROS of OH radicals were generated. This new ROS generation method was combined with microchannel cell culture system for analyzing ROS effects on cell culture. The system developed here opens a new way for affecting cell culture and elucidating ROS effects on cells.