The Microscopic Biopsy Device for the Highly Viscoelastic Tissue by Using Translational/Rotational Piezo Impact Drive Mechanism

A microscopic biopsy device utilizing a trans-lational/rotational piezoelectric impact drive Mechanism (Piezo IDM) was developed for ultra-precise cell extraction from highly viscoelastic biological tissue. To correlate local gene expression with tissue microstructures at the micrometer scale, the device employs a knife-edged glass capillary for localized tissue sampling. A novel rotational impact motion was introduced to enhance suction force, enabling successful extraction of target tissues that were previously unattainable with conventional methods. The device was put into practical use for neutrophil isolation experiments in thrombus-induced mouse, demonstrating its capability for precise tissue extraction in vivo.