Genetic determinants of pOXA-48 plasmid maintenance and propagation in Escherichia coli
Conjugative plasmids are the main drivers of antibiotic resistance dissemination contributing to the emergence and extensive spread of multidrug resistance clinical bacterial pathogens. pOXA-48 plasmids, belonging to the IncL group, emerge as the primary vehicle for carbapenem resistance in Enterobacteriaceae. Despite the problematic prevalence of pOXA-48, most research focus on epidemiology and genomics, leaving gaps in our understanding of the mechanisms behind its propagation. In this study, we use a transposon sequencing approach to identify genetic elements critical for plasmid stability, replication, and conjugative transfer. Our results identify a novel type I toxin-antitoxin system, uncharacterized essential maintenance factors, and components of the type IV secretion system and regulatory elements crucial for conjugation. This study advances our understanding of pOXA-48 biology, providing key insights into the genetic factors underlying its successful maintenance and spread in bacterial populations.
Authors
- Julie Baltenneck (ORCID: https://orcid.org/0000-0001-8456-4126)
- Annick Berne-Dedieu (ORCID: https://orcid.org/0000-0002-9982-8793)
- Bogdan Iuliu Iorga (ORCID: https://orcid.org/0000-0003-0392-1350)
- Christian Lesterlin (ORCID: https://orcid.org/0000-0002-9108-0848)
- Jonathan Degosserie (ORCID: https://orcid.org/0000-0001-8654-4831)
- Pierre Bogaerts (ORCID: https://orcid.org/0000-0002-4016-3707)
- Sarah Bigot (ORCID: https://orcid.org/0000-0002-9340-6303)
- Marie‐Eve Val (ORCID: https://orcid.org/0000-0001-7097-9072)
- Nathan Fraikin (ORCID: https://orcid.org/0000-0002-0329-5313)
- Erwan Gueguen (ORCID: https://orcid.org/0000-0001-8784-1491)
- Yannick Baffert
- Yasmine Makhloufi
Institutions
- Université Claude Bernard Lyon 1 (FR)
- Centre National de la Recherche Scientifique (FR)
- Institut de Chimie des Substances Naturelles (FR)
- Inserm (FR)
- Institut Pasteur (FR)
- Université Paris Cité (FR)
- Université Paris-Saclay (FR)
- CHU Dinant Godinne UCL Namur (BE)
- Structural and Molecular Basis of Infectious Systems (FR)
- Laboratoire de Microbiologie, Adaptation et Pathogénie (FR)
- Institut National des Sciences Appliquées de Lyon (FR)
Publication Details
- Journal
- Nature Communications
- Published
- 2025-08-19
- DOI
- https://doi.org/10.1038/s41467-025-62404-7
- Citations
- 4
- Primary Topic
- Antibiotic Resistance in Bacteria
- Type
- article
- Field-Weighted Citation Impact
- 2.36
Funders
- Agence Nationale de la Recherche
- Fondation pour la Recherche Médicale
- Université de Lyon