RAW 264.7 cell phagocytosis assay v1
This protocol describes in vitro methods for evaluating phagocytosis using the macrophage-like cell line, RAW 264.7. The two methods discussed here include (1) a photographic phagocytosis assay, which is compatible with any target that is visible via 100x microscopy, and measures total uptake of target material per macrophage, and (2) the phagocytosis-kill assay, which measures killing of phagocytosed microbial targets by the macrophages.
Authors
- Brian M Luna (ORCID: https://orcid.org/0000-0001-6718-4551)
- Travis B. Nielsen (ORCID: https://orcid.org/0000-0003-3366-7912)
- Brad J. Spellberg (ORCID: https://orcid.org/0000-0002-3632-1709)
- Donghong Yu (ORCID: https://orcid.org/0000-0002-3035-5035)
- Matthew Slarve
- Jordyn VanOrman (ORCID: https://orcid.org/0009-0009-1240-7477)
Institutions
- California Southern University (US)
- Southern States University (US)
Publication Details
- Published
- 2025-06-05
- DOI
- https://doi.org/10.17504/protocols.io.yxmvmmr16v3p/v1
- Primary Topic
- Phagocytosis and Immune Regulation
- Type
- article
- Field-Weighted Citation Impact
- 0.00